Post Challenge survival.

Groups of 5 A/J mice immunised with a DC vaccine (comprising of DCs stimulated overnight with rPA, heat-killed B. anthracis and CpG), rPA in alum, or rPA in alum plus DC vaccine, or left naïve, were challenged at 14 days post-immunisation with 3×10⁴ CFU B. anthracis STI (i.p.) and monitored for survival over the subsequent 8 days. There was a statistically significant difference in the survival curves for the negative control (alum+BMDC&CpG) group compared with the group actively immunised with rPA& alum and also receiving rPA&HK B.anthracis-pulsed DC (p<0.002) by both the Mantel-Cox and Gehan-Breslow-Wilcoxon tests. Comparison of survival curves between the negative control (alum+BMDC&CpG) group and the group receiving rPA&HK B.anthracis-pulsed DC only, was also statistically significant (p<0.01) when both tests were applied.