Porcine ROSA26 gene targeting using TGROSA construct.
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posted on 2014-07-15, 02:59 authored by Shun Li, Tatiana Flisikowska, Mayuko Kurome, Valeri Zakhartchenko, Barbara Kessler, Dieter Saur, Alexander Kind, Eckhard Wolf, Krzysztof Flisikowski, Angelika Schnieke(A) Porcine ROSA26 gene targeting scheme. Top. Porcine ROSA26 gene. Exon numbers are indicated. Below. TGROSA gene targeting vector. PCR and RT-PCR primers used to identify targeted cell cones and detect mRNAs are indicated. SbfI restriction sites and the hybridisation probe used for Southern analysis are also shown. (B) Southern blot analysis of TGROSA targeted porcine ROSA26. Lanes show SbfI digested genomic DNA from three TGROSA targeted MSC clones 4, 6 and 18, four foetuses derived by nuclear transfer, newborn piglet 131 and a wild-type piglet as indicated. The 5783 bp diagnostic fragment detected by the bsr probe is shown. (C) Nuclear transfer derived TGROSA piglet (left) and wild-type piglet (right).
