Porcine ROSA26 gene targeting using GCROSA construct.
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posted on 2014-07-15, 02:59 authored by Shun Li, Tatiana Flisikowska, Mayuko Kurome, Valeri Zakhartchenko, Barbara Kessler, Dieter Saur, Alexander Kind, Eckhard Wolf, Krzysztof Flisikowski, Angelika Schnieke(A) Top. Porcine ROSA26 gene. Exon numbers are indicated. Below. GCROSA gene targeting vector. PCR primers used to identify targeted MSC clones are indicated. (B) 5′ junction PCR of five representative GCROSA targeted MSC. Predicted amplified fragment size is 2630 bp. (C) 3′ junction PCR of five representative GCROSA targeted MSC. Predicted amplified fragment size is 6331 bp. (D) lacZ staining of reporter constructs. a1–a3, black and white channel. b1–b3, non-filtered colour channel. c1–c3, colour channel with grey filter. Top panels, GCROSA targeted MSCs; middle panels, MSCs transfected with CAG promoter directed β-geo construct; bottom panels, wild type MSCs.
