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Platelet adhesion to a panel of ovarian cancer cell lines is heterogeneous under static conditions.

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posted on 2011-10-12, 01:31 authored by Karl Egan, Darragh Crowley, Paul Smyth, Sharon O'Toole, Cathy Spillane, Cara Martin, Michael Gallagher, Aoife Canney, Lucy Norris, Niamh Conlon, Lynda McEvoy, Brendan Ffrench, Britta Stordal, Helen Keegan, Stephen Finn, Victoria McEneaney, Alex Laios, Jens Ducrée, Eimear Dunne, Leila Smith, Michael Berndt, Orla Sheils, Dermot Kenny, John O'Leary

[A] Platelet adhesion to ovarian cancer cells was quantified based on the fluorescence detection of labelled platelets. Platelet adhesion to fibrinogen and BSA were used as ± controls [n = 8 + SEM, *  =  p<0.05 vs. BSA]. Fluorescence microscopy images of platelets adhering to A2780 [B] and 59M [C] cells under static conditions [representative of n = 3]. Ovarian cancer cells and platelets were stained for actin [green], platelets were stained specifically for CD42a [red/yellow].

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