Public Library of Science
Browse
Figure_4.tif (1.76 MB)

Piperine induced caspase activation in prostate cancer cell lines.

Download (0 kB)
figure
posted on 2013-06-18, 01:14 authored by Abhilash Samykutty, Aditya Vittal Shetty, Gajalakshmi Dakshinamoorthy, Mary Margaret Bartik, Gary Leon Johnson, Brian Webb, Guoxing Zheng, Aoshuang Chen, Ramaswamy Kalyanasundaram, Gnanasekar Munirathinam

A NIR-FLIVO 747-conjugated poly-caspase probe, which is a cell-permeable fluorescent detector of active caspases, was employed to determine whether piperine activates caspase in prostate cancer cells. The LNCaP and PC-3 cell lines were treated with piperine and then tested for caspase activation. Results showed that piperine induced global caspase activation in both LNCaP (A) and PC-3 cells (B) as early as 24 hours. Experiments were repeated four times and obtained similar results as shown in this representative figure.

History