Phosphomonoester region of ³¹P-NMR spectra of ethanol extracts obtained in late-exponential cells of S. pneumoniae D39 grown in CDM with galactose (Gal), mannose (Man) or N-acetylglucosamine (GlcNAc).

Ethanol extracts of glucose (Glc) grown cells were used as control. The glycolytic intermediates fructose 1,6-biphosphate (FBP), glucose 6-phosphate (G6P) and 3-phosphoglycerate (3-PGA) were firmly assigned. Abbreviations: αGal1P, α-galactose 1-phosphate; αGlc1P, α-glucose 1-phosphate; TBP, tagatose 1,6-diphosphate; Gal6P, galactose 6-phosphate; Man6P, mannose 6-phosphate; GlcN6P, glucosamine 6-phosphate; GlcNAc6P, N-acetylglucosamine 6-phosphate and F6P, fructose 6-phosphate. Identification of phosphorylated intermediates was performed by spiking the ethanol extracts with pure compounds. Symbols: (asterisk) FBP, (closed square) G6P, (open diamond) 3-PGA.