Pathway schematic depicting changes that occurred in measured metabolites, gene expression, and enzymatic activities for central metabolism during the transition from succinate to methanol growth.

A boxed gene/protein name indicates the activity of this enzyme was measured. Red lettering for the protein designation indicates an increase in activity; green, decrease; black, no change. Metabolites appearing more than once are connected by gray lines. Changes are shown for (A) the initial response, time  = 10–30 min with (B) serving as a legend. Graphs depicting changes for pre-methanol addition, time  = 0 min; just prior to/at the start of cell growth, time  = 1–2 h; and exponential cell growth, time  = 3–6 h are included in Figure S1. Reaction descriptions are included in Table S2 along with gene expression intensities, LogRatios, fold changes and p-values. Mesaconyl-CoA, ethylmalonyl-CoA, methylsuccinyl-CoA were measured as free acids. **Color at T = 0 represents the concentration before methanol was added for all metabolites except for methanol which was calculated as 50 mM for the initial T = 0 value.