PTPN14 is a YAP-binding protein.
figure
posted on 2013-04-16, 00:54 authored by Chrysiis Michaloglou, Waltraut Lehmann, Typhaine Martin, Clara Delaunay, Andreas Hueber, Louise Barys, Honglin Niu, Eric Billy, Markus Wartmann, Moriko Ito, Christopher J. Wilson, Mary Ellen Digan, Andreas Bauer, Hans Voshol, Gerhard Christofori, William R. Sellers, Francesco Hofmann, Tobias SchmelzleA) QPCR-based YAP copy number analysis. MCF-10A is used as a control cell line with no YAP amplification. B) Clonogenic survival assay of SF268 cells transduced with lentivirus encoding control or two independent dox-inducible shRNAs (upper panel). QRTPCR analysis of YAP mRNA levels (lower panel). C) Coomassie-stained gel used for MS analysis of YAP-binding proteins. YAP is indicated by the black arrow, PTPN14 by the black arrowhead and the AMOT family members by an asterisk. D) IP of endogenous YAP from SF268 cell lysates. All lanes are from a single blot and exposure. Asterisk indicates the position of the IgG heavy chains.
