PDF-1 secretion is not altered in glr-2 mutants.

PDF-1 secretion was analyzed in the indicated genotypes. YFP-tagged PDF-1 was expressed with the pdf-1 promoter. Representative images (A) and summary data (cumulative fraction) (B-C) are shown for coelomocyte fluorescence in L4/A lethargus and 1-day old adults of the indicated genotypes. PDF-1::YFP coelomocyte fluorescence was dramatically increased in npr-1 mutants during the L4/A lethargus and in adults as previously reported [11]. Mutations inactivating GLR-2 did not alter PDF-1::YFP coelomocyte fluorescence during L4/A lethargus (B) and in adults (C) in either wild type or npr-1 mutants. Scale bar indicates 10 μm. p values are indicated for each comparison (Kolmogorov-Smirnov test).