Overview on ErbB4 expression in nucleus accumbens, dorsal striatum and surrounding cortex.

(A) Single epifluorescence images (868) of a frontal lobe section double-labeled for ErbB4 (mAb-77, green channel) and TH (red channel) were taken at 10x magnification and automatically assembled. The drawing indicates the approximate anteroposterior position of the section (red line). Immunofluorescence of TH highlights the Cd, Pu, NAC and OTu. ErbB4 expression is evident in all cortical areas; however, immunoreactivity is stronger in dorsal and lateral cortex compared to medial and ventral areas. ErbB4 is expressed at medium levels in the striatum with patches of higher immunofluorescence being evident in the NAC and OTu. The location of areas of interest shown at higher magnification in panels B and C is indicated by rectangles. (B) ErbB4 expression is restricted to small multipolar neurons (arrows) in deep layers of cortical area 6, whereas large, fuzzy areas of high intensity (C) occur at the border of OTu and NAC in the ventral forebrain. Scale bar = 2 mm (A), 220 µm (B, C). Abbreviations: Supplementary motor area and premotor cortex (6), anterior cingulate cortex (24), subgenual prefrontal cortex (25), caudate (Cd), claustrum (Cl), cortical layers (L5, L6), nucleus accumbens (NAC), olfactory tubercle (OTu), putamen (Pu).