Overexpression of Myc increases mutation frequency and decreases lifespan at 29

°C. (A) Western blot from five adult heads at 29°C for three days from lacZ #2/Tub-Gal80TS; Actin-Gal4/+ (shown as ActTS>+) and lacZ #2/Tub-Gal80TS; Actin-Gal4/UAS-Myc (shown as ActTS>Myc). Myc is induced ∼9-fold in this system (LiCOR quantitation). (B) lacZ mutation frequency of lacZ #2/Tub-Gal80TS; Actin-Gal4/+ (shown as “ActTS>+”) and lacZ #2/Tub-Gal80TS; Actin-Gal4/UAS-Myc (shown as “ActTS>Myc”). Adults were placed at 29°C for 5 days before lacZ analyses to allow transgene expression. Black solid areas indicate frequency of point mutations and white areas indicate frequency of genome rearrangements. *indicates statistical significance of p<0.01 (student’s t-test). (C) Lifespan analyses of females of the genotype lacZ #2/Tub-Gal80TS; Actin-Gal4/+ (shown as “ActTS>+”) and of the genotype lacZ #2/Tub-Gal80TS; Actin-Gal4/UAS-Myc (shown as “ActTS>Myc”). These lifespans are statistically significantly different from each other p<0.001 (Log-rank test).



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