Overexpression of AtLOV1 caused erect leaf phenotype in switchgrass.

(A) Transgenic plants had smaller leaf angles compared to wild type (WT). (B) The leaf angles of four independent T0 transgenic lines were associated with the expression of AtLOV1 in plants as detected by RT-PCR (noting that AtLOV1 pre-mRNA could be alternatively spliced in switchgrass, yielding the transcripts AtLOV1.1 and AtLOV1.2). Different letters above the bars indicate statistically significant differences (α = 0.01). (C) Southern blot analysis with an hph probe showed T-DNA integration in five T0 transgenic lines.