Figure_6.tif (5.06 MB)
Osterix affects NELL-1 and some bone marker genes expression in Saos-2 cells (A) and primary human osteoblast (hOB) (B).
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posted on 2013-02-20, 14:53 authored by Feng Chen, Xinli Zhang, Shan Sun, Janette N. Zara, Xuan Zou, Robert Chiu, Cymbelin T. Culiat, Kang Ting, Chia SooReal time PCR analysis of Nell-1, OCN and OPN transcripts in Saos-2 cells transiently transfected with Control (pCtr) or Osterix expression vector (pOsx) at 2 days and 7 days (top panel). The effects with transcient transfection of Negative control siRNA (NC-siRNA) or Osterix siRNA mixture (Osx-siRNA) were revealed by Real time PCR analysis at 2 days and 7 days (bottom panel). (C) Diagram of the regulatory relationship between Runx2, Osterix and NELL-1. Runx2 positively regulates Osterix and NELL-1. Osterix negatively controlled NELL-1 expression in this study. NELL-1 was also shown to positively affect Runx2 through phosphorylation and negatively feedback on Osterix during osteogenesis.