Origin and fate of cerebellar ectopia.

(A) Rare ectopia continuous with the EGL, H & E staining. (B) Image of rare Math1⁺ ectopia continuous with the EGL in GFAP:Hi-Otx2, MATH1-GFP mice. (C–D, F–M) Images of GFAP:Hi-Otx2 brain sections stained with the indicated antibodies. (C–D) Sections immunostained with Ki67 and Zic1 of (C) rare ectopia continuous with the EGL and (D) representative ectopia located just deep to the IGL. (E) Localization of histologically-apparent ectopia of GFAP:Hi-Otx2 mice at P3 and P7 (distance in µm). (F, G) Expression of differentiation markers in cerebellar ectopia at (F) P7 and (G) P21. (H–K) Location of partially- to fully-differentiated neurons in (H, I) cerebellum or (J, K) brainstem as evidenced by Zic1 and Ki67 staining shown at 10× mag in (H, J) wild type mice or (I, K) GFAP:Hi-Otx2 mice. (L, M) Cleaved caspase-3 staining of ectopia in the (L) cerebellum and (M) brainstem. All images except (H–K) shown at 20× mag. CB, cerebellum; BS, brainstem. Asterisk indicates p≤0.05 relative to P3, student's t-test. Arrows indicate ectopia. Boxes indicate location of cropped and zoomed image.