One-step viral growth curves of human wt-RV and r-RV.
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posted on 2013-02-20, 18:24 authored by Cécile Troupin, Aurélie Schnuriger, Sarah Duponchel, Claire Deback, Nathalie Schnepf, Axelle Dehee, Antoine Garbarg-ChenonFor each virus, confluent MA-104 cells were inoculated at the same MOI of 0.5 PFU/cell. The infected cell cultures were harvested at 2, 6, 8, 10, and 18 hours post-infection and virus titers were determined by plaque assay in MA-104 cells. Human wt-RV M0 and r-RV M1 to M4 share the same genetic background. The rearranged segments of r-RV M1 to M4 are indicated in brackets. The bovine RV strain RF was used as control.
