One or two day stress does not have significant effects.

A) To control for effects due to a single stress or two days of stress, and not due to the cumulative effects of stress, the effects of one (top) or two days (bottom) of stress was examined. Solid bars indicate the day of stress, open bars indicate days of no stress, oval indicates the test day. B) Neither one nor two days of stress had any significant effects on body weight over the course of the experiment, nor on body weight one day after the final stress, compared to control handling. C) Neither one nor two days of stress had any significant effects on adrenal gland weight.