Oil Red O Staining and quantification on Day 7.
figure
posted on 2015-02-25, 03:57 authored by Malini Visweswaran, Luca Schiefer, Frank Arfuso, Rodney J. Dilley, Philip Newsholme, Arun Dharmarajan(A) Microscopic observations of the stained lipid droplets. Treatment conditions were (i) non-induced control, (ii) adipogenic control, (iii) 10mM LiCl, (iv) 0.5μM BIO, (v) 100pg/mL sFRP4, and (vi) 1ng/mL sFRP4. Scale bar = 250μM (B) Quantification of the stained lipid droplets were performed using the eluted Oil red O stain via measuring absorbance at 510nm. The readings were normalised to background values of non-induced control ADSCs. The values of all treatment conditions were compared to the adipogenic control group (* p<0.05 and ** p<0.001).
