Ofd2 interacts with histones.
(A) Dot blot analysis. Calf thymus histones (ctH2A, ctH2B, ctH3 and unfractionated whole histones), BSA and Ofd2 were dotted onto a PVDF membrane and probed with Ofd2 (left panel) or with no protein (right panel). Protein complexes were visualized with chemiluminescence. (B) Far western analysis. Unfractionated calf thymus histones, recombinant histones (rH2A, rH2B, rH3, rH4) and Ofd2 were separated on a 12% SDS-PAGE, transferred to a PVDF membrane and probed with Ofd2 (upper panel) or with no protein (middle panel). A third gel was stained with Coomassie Blue to visualize the proteins (lower panel).