Obtained diversity by OmniChange shown as partial sequence alignment of 48 randomly “picked” clones.

Partial sequence alignment of wild-type gene (template DNA) to 48 random clones from a multi site-saturation library generated by OmniChange. The five sites targeted for saturation are highlighted in white. Arrows indicate the distance between the targeted positions in base pairs. A comparison of the codons in the white columns shows the obtained diversity by saturating five independent positions simultaneously by OmniChange.