OTC enzyme and biochemical parameters in spf-J males.

Spf-J and WT were maintained on a regular chow diet (22% protein) and euthanized at 8 weeks. Blood was collected by retro-orbital bleeding with plasma separated and stored at −80°C until use. Tissues were snap frozen and stored at −80°C until use. A) mRNA level in spf-J (N = 4) and WT (N = 4) livers. B) Immunoblot of OTC protein. C) OTC enzyme activity in liver homogenates from spf-j (N = 6) and WT (N = 4). D) Plasma ammonia in spf-j (N = 7) and WT (N = 6). E) Plasma orotate in spf-j (N = 5) and WT (N = 6). Hatched bar indicates P < 0.05. WT – wild-type.