OCI-AML3 cells transduced with LC3-GFP-mCherry construct were treated with 5 μM Nutlin 3a and visualized by confocal microscopy.

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posted on 2015-10-06, 04:11 authored by Gautam Borthakur, Seshagiri Duvvuri, Vivian Ruvolo, Durga Nand Tripathi, Sujan Piya, Jared Burks, Rodrigo Jacamo, Kensuke Kojima, Peter Ruvolo, Juan Fueyo-Margareto, Marina Konopleva, Michael Andreeff

Increase in proportion of red ‘puncta’ at 96 hrs compared to 48 hrs (merge images) in Nutlin 3a treated cells indicate completion of autophagic flux.

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AMP Kinase Activation MDM 2 cell death pathways manner autophagy impairs apoptosis Nutlin 3 leukemia MDM 2 inhibitor p 53 ampk BECLIN 1 knockdown Induces p 53 Dependent Autophagy inhibitor

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OCI-AML3 cells transduced with LC3-GFP-mCherry construct were treated with 5 μM Nutlin 3a and visualized by confocal microscopy.

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