Northwestern blot assay of RNA-binding by mTERF15 recombinant protein.

The first panel shows Coomassie brilliant blue (CBB) staining of MBP-strep and mTERF15-strep lacking the first 34 N-terminal amino acids. The second panel shows immunoblot analysis with anti-strep antibody to recognize the strep-tag. The third panel shows the northwestern blot analysis using radioisotope-labeled total RNA isolated from 7-d-old seedlings. The final panel shows the northwestern blot analysis using radioisotope-labeled nad2 intron 3; signals are observed on the PVDF membrane corresponding to the RNAs bound to mTERF15.