posted on 2013-06-06, 01:03authored byXiufeng Wu, Gang Chen, Jianping Lu, Weifeng Zhu, Jingting Qiu, Jianxin Chen, Shusen Xie, Shuangmu Zhuo, Jun Yan
Multiphoton microscopy (MPM) image (A) and corresponding hematoxylin-eosin (H-E) image (B) at low magnification show normal breast is predominantly consist of adipose and fibrous structures surrounding ducts. The MPM image (C) and corresponding H-E image (D) at high magnification show ducts are lined by simple columnar epithelium and basement membrane. In MPM image, the red color represents the SHG signal from collagen, and the green color represents TPEF from autofluorescence in cells. (Original magnifications 63x [A, C]; 20x [B]; 40x [D]).