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Non-Leukemogenic Forms of ICN Fail to Rescue 8946 Cell Line Growth and do not Transactivate Myc.

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posted on 2011-10-13, 01:40 authored by Jon C. Aster, Nick Bodnar, Lanwei Xu, Fredrick Karnell, John M. Milholland, Ivan Maillard, Gavin Histen, Yunsun Nam, Stephen C. Blacklow, Warren S. Pear

(A) Comparison of rescue of 8946 cells by various ICN polypeptides following doxycycline-mediated withdrawal of MYC. 8946 cells were transduced with MigRI viruses, sorted for GFP positivity, and then treated with doxycycline (20 microgram/ml) for up to 8 days. Cell numbers at each time point are shown. (B, C) 8946 cells transduced as in (A) were harvested after 24 hr of treatment with doxycycline (20 microgram/ml) or vehicle (PBS). Levels of Myc (B) and Deltex1 (C) mRNAs were determined by qRT-PCR.

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