Public Library of Science
Browse
Figure_6.tif (1.41 MB)

Nkx3.2 and Sox9 can induce muscle satellite cells to express cartilage markers collagen II and aggrecan.

Download (0 kB)
figure
posted on 2012-07-02, 01:37 authored by Dana M. Cairns, Renjing Liu, Manpreet Sen, James P. Canner, Aaron Schindeler, David G. Little, Li Zeng

Muscle satellite cells were infected with RCAS viruses encoding GFP, Nkx3.2HA, Sox9V5 or Nkx3.2HA+Sox9V5. Immunocytochemistry and qRT-PCR analyses were performed. For all qRT-PCR, GAPDH was used for normalization. (A) Immunocytochemistry analysis of collagen II protein expression in satellite cells. Virus staining images were overlaid with collagen II images, and DAPI stains cell nuclei. (B) Western Blot analysis of collagen II protein expression in satellite cells. ß-actin, internal control. (C) qRT-PCR analysis of collagen II mRNA and (D) Aggrecan mRNA expression. (E) Glycoaminoglycan (GAG) assay. “*” denotes p<0.05 in statistical analysis.

History

Usage metrics

    PLOS ONE

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC