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Neutrophil recruitment following L. braziliensis inoculation.
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posted on 2015-03-10, 02:44 authored by Sarah A. C. Falcão, Tiffany Weinkopff, Benjamin P. Hurrell, Fabiana S. Celes, Rebecca P. Curvelo, Deboraci B. Prates, Aldina Barral, Valeria M. Borges, Fabienne Tacchini-Cottier, Camila I. de OliveiraIndividual BALB/c mice were injected with L. braziliensis in the ear dermis. At different time points, cells were prepared and stained for Ly6G and analyzed by flow cytometry. (A) Representative dot plots and gating strategy of neutrophils. (B) Bar graph representation of the number of Ly6G+ cells in naïve mice (grey bars), in mice inoculated with saline (white bars) or inoculated with L. braziliensis (black bars). Data shown (mean ± SEM) are from one experiment representative of two. ***p <0.001, *p<0.05.
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parasite loadneutrophil recruitmentneutrophil accumulationparasite eliminationCell activationbraziliensis promastigotesCD 62Lapoptotic fate.Methodssurface moleculesLeishmania inoculationapoptosis.ConclusionsWe showbraziliensis exposureoxidative burstneutrophil elastaseCD 18Leishmania braziliensis infectionNeutrophil activationbystander cellsbone marrow neutrophilsApoptosis BackgroundNeutrophils24 hLeishmania braziliensis Triggers Neutrophil Activationtnf
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