Neutrophil accumulation and myeloperoxidase (MPO) activity in the lungs after hemorrhagic shock and resuscitation (HSR).

Lungs from the HSR group rats treated with or without biliverdin (BV) were excised 12 h after resuscitation. Neutrophils were stained by the naphthol AS-D chloroacetate method and MPO activity was measured. (A) Each photograph is representative of five independent experiments. The arrows indicate positively stained neutrophils (original magnification ×400, scale bar  = 50 µm). (B) The number of neutrophils in five nonconsecutive lung sections per rat at a magnification of ×400 (n = 5 per group). (C) Lung MPO activity (n = 6 per group). Data are presented as means ± standard deviation and were statistically evaluated using analysis of variance followed by Tukey–Kramer honestly significant difference test. *p<0.05 vs. vehicle/sham; p<0.05 vs. BV/sham; #p<0.05 vs. vehicle/HSR. Vehicle/sham, vehicle-administered animals subjected to sham surgery; BV/sham, BV-administered animals subjected to sham surgery; vehicle/HSR, vehicle-administered animals subjected to HSR; BV/HSR, BV-administered animals subjected to HSR.