Nestin-Cre mediated deletion of braf impairs neuronal differentiation in the granular cell layer of the dentate gyrus.

(A) Quantification of BrdU-labelled cells in the dentate gyrus of ctrl or cKO mice. Cells were labelled in vivo with BrdU at days P10 and P11, followed by sacrification 24 hours after the second BrdU pulse. Representative sagittal sections of the dentate gyrus stained with the proliferation marker BrdU (green). Data are mean ±s.e.m.; n = 4. Scale bar = 50 µm. (B) Quantification of BrdU-labelled cells in the dentate gyrus at P22 of ctrl or cKO mice. Neural progenitor cells were labelled in vivo with BrdU at days P10 and P11, followed by sacrification of mice at P22. Representative sagittal sections of the dentate gyrus stained with proliferation marker BrdU (green). Data are mean ±s.e.m.; n = 4. Scale bar = 50 µm. (C) Quantification of BrdU/NeuN-positive cells in the granular cell layer of the dentate gyrus of ctrl cKO mice. Neural progenitor cells were labelled in vivo with BrdU at days P10 and P11, followed by sacrification of mice at P22. Representative sagittal sections of the dentate gyrus stained with proliferation marker BrdU (green) and neuronal marker NeuN (red) 11–12 days after BrdU labelling. Double positive cells are marked with an arrow. Data are mean ±s.e.m.; n = 4. Scale bar = 50 µm. (D) Quantification of BrdU/GFAP-positive radial glia cells in the granular cell layer of the dentate gyrus of ctrl or cKO mice. Neural progenitor cells were labelled in vivo with BrdU at days P10 and P11, followed by sacrification of mice at P22. Representative sagittal sections of the dentate gyrus stained with proliferation marker BrdU (green) and neural precursor/astrocyte marker GFAP (red) 11–12 days after BrdU labelling. Expanded region is indicated by an arrow; the arrowhead depicts a double-positive cell. Data are mean ±s.e.m.; n = 4. Scale bar = 50 µm.