Nemitin is enriched in the nervous system.

(A) The presence of nemitin transcripts was surveyed in adult mouse tissues by reverse transcriptase-PCR. Nemitin mRNA was observed in the brain, heart, kidney, liver, and lung, with the highest expression in the brain. (B) The presence of nemitin protein in adult mouse tissues was visualized by western blot using the nemitin antibody and a β-actin antibody as loading control. Nemitin was found in all brain regions tested (left panel). By contrast, of the other tissues examined, only the lung and testes showed appreciable expression of nemitin (middle panels). Endogenous nemitin protein was detected in cultured cortical neurons as well as the neuronal PC12 cell line, but little or no expression was observed in COS7 cells (right panel). (C) Immunostaining of an E14 mouse embryo using nemitin and MAP2 antibodies shows that nemitin is expressed in the brain and spinal cord (C-a) and that its expression correlates closely with MAP2 (C-b). Inset shows appreciable expression of both nemitin and MAP2 in dorsal root ganglia.