Negative-stain electron microscopic (EM) image classificaion of the EBV gHgL/gp42/HLA-DQ2 triggering complex.
(A) A representative negative-stain EM image of gHgL/gp42/HLA-DQ2 sample. (B) Representative class averages of gHgL/gp42. Shown to the side is a schematic representation depicting the variability in the gp42 CTLD position along the length of gHgL before HLA-class II binding. (C) Representative class averages of gHgL/gp42/HLA-DQ2 complexes in open and closed conformations in ∼50∶50 proportion. The particle numbers included in the ternary complex classes are also indicated in each class average in (C).