NCM-mediated protection of brain cells against GOSD-induced cell injury was interrupted by antibodies specific for TGFβ1, GDNF or NT-3.

NCM collected from the neuronal culture (6.4x10⁶/dish in 10-cm dish plates) under the GOSD condition for 6 h was pre-incubated with anti-TGFβ1, anti-GDNF, anti-NT3 or normal rabbit sera (NRS) for 1 h before adding it to microglia (A, B and C), astrocytes (D) and neurons (E) and then followed by co-incubation for 2 h (microglia) or 6 h (astrocytes and neurons), under the GOSD condition. Number of the surviving cells in each group was determined by trypan blue dye exclusion assay. ^aSignificant difference between lane 1 and the indicated lanes; ^b between lane 2 and the indicated lanes; ^c between lanes 5 and 6, p<0.05. Each experiment was repeated for 3 times.