NCI-H28 and REN cells express significant less amount of PAR1 on plasma membrane.

Cell surface PAR1 expression was measured by ELISA using a polyclonal antibody which recognizes the N-terminus of PAR1[31]. Antibody binding to fixed cells was detected by horseradish peroxidise-conjugated secondary antibody. Data represent the mean ± SEM of three independent experiments performed in triplicate. The differences in cell surface PAR1 expression between Ctrl (Met-5A cells) and MPM (NCI-H28 and REN) cellswere significant (***P≤0.001) by one-way ANOVA followed by Bonferroni’s multiple comparison test.