Multiple sequence alignment showing conserved regions of Mps1p homologs.

(A) Alignment of N-terminal region of Mps1, showing conserved Leucine residue. (B) Sequence alignment to show C-terminal homology of CaMps1 (Orf19.7293/AY632356), Mps1 of S. cerevisiae (YDL028C/P54199), Mph1 of S. pombe (O94235), hMps1 of Human (Q9BW51) and mMps1 of Mouse (P35761): Where ORF numbers were not obtained, accession numbers are provided. Eleven subdomains are shown in Roman numerals (I–XI). Subdomains of the kinase domain are shown as described by [30]. The conserved amino acids in serine/threonine kinases (SER/THR) are shown as described by [31], conserved amino acids are shown in bold letters. The italicized E in the sub domain V is not part of the SER/THR consensus, but a feature of dual specificity kinases [31]. Valine (V) at position 382 is the site of insertional inactivation. Well conserved aa are indicated with a double dot (:) and the perfectly conserved aa are indicated with an asterisk (*)