Mover is a homomeric protein.
figure
posted on 2013-05-28, 02:03 authored by Saheeb Ahmed, Nina Wittenmayer, Thomas Kremer, Jan Hoeber, Asha Kiran Akula, Henning Urlaub, Markus Islinger, Joachim Kirsch, Camin Dean, Thomas Dresbach(A) Yeast 2-hybrid assay using full length Mover (full-l.) as bait. A plasmid containing only the LexA interaction domain acted as a control. Beta-galactosidase expression was only induced if full-length Mover was used as both prey (rows) and bait (columns). All deletion constructs used as prey exhibited no interaction with full-length Mover. (B) Immunoprecipitation of GFP-Mover with Mover-myc co-expressed in HEK293 cells. Sepharose-coupled antibodies against the myc epitope were used to pull down protein complexes. As in the yeast 2-hybrid assay, only full-length GFP-Mover was pulled down from the HEK cell extracts, whereas GFP and GFP-Mover deletion constructs were not. N = 2 experiments.
