Molecular marker analysis of Mrotm1H homozygous embryonic male gonads.
(A) Wholemount in situ analysis of developing male gonads (13.5 dpc) explanted from wildtype (+/+) and homozygous mutant (−/−) embryos using a probe for the Sertoli cell marker Sox9. (B, C) transverse sections through the gonads reveals expression in the testis cords, with signal concentrating at the periphery of the cords. (D) Analysis of expression of the germ cell marker Oct4. (E, F) Expression is observed in the testis cords, the signal being restricted to large round germ cells in the centre of the cords. (G) Analysis of expression with the Leydig cell marker 3βHSD. (H, I) Expression is restricted to cells of the interstitium. No significant differences between wild-type and mutant samples is observed with any of these markers. Scale bars show 50 µM.
