Figure_1.tif (2.29 MB)
Molecular characterization of transgenic rice lines expressing AhSTS1.
figure
posted on 2013-03-05, 09:47 authored by So-Hyeon Baek, Woon-Chul Shin, Hak-Seung Ryu, Dae-Woo Lee, Eunjung Moon, Chun-Sun Seo, Eunson Hwang, Hyun-Seo Lee, Mi-Hyun Ahn, Youngju Jeon, Hyeon-Jung Kang, Sang-Won Lee, Sun Yeou Kim, Roshan D’Souza, Hyeon-Jin Kim, Seong-Tshool Hong, Jong-Seong Jeon(A) Southern blot analysis. Genomic DNA in lanes P and RS1 to RS22 were digested with BamHI (specific to the T-DNA region). The arrow indicates the fragment (1.2 kb) hybridized with the AhSTS1 cDNA probe. P, pSB2220 vector; N, non-transgenic wild-type Dongjin; lanes RS1 - RS22, representative transgenic Dongjin lines out of 129 T1 samples. (B) RT-PCR analysis. Total RNA from leaf samples of the same lines as in (A) was analyzed. OsUBQ5 was included as a PCR control.
