Modulation of AMPK/ACC pathway in central nervous system of ACC-ASO and ACC-SO mice.

Representative western blot of hypothalamic p-AMPK (A) and ACC (B) in fasted and fed rats, ACC-ASO (ASO) and ACC-SO (SO). Representative western blot of hippocampus and brainstem area of ACC (C). Representative ACC immunoflorescence staining (green) of samples from hypothalamus (ARC, LH and PVH) of Wistar rats. Cell nuclei were counterstained with DAPI (blue) (D). Bars show quantification of total p-AMPK and ACC proteins normalized by either total GAPDH or AMPK. Data are means ± SEM of five rats. (A)*p≤0.05 vs. control fed, ASO and SO. (B)*p≤0.05 vs. control and SO.