MmmSC-induced recall IFN-γ responses measured by ELISA (a) and ELISPOT (b) using pbmc collected from nonvaccinated (open circles) and vaccinated (closed circles) animals (n = 5) one month after single, double and triple vaccine inoculations.

Results represent the net effect of MmmSC (i.e., stimulated cultures minus non-stimulated cultures) and are representative of two experiments. Bars indicate median values and asterisks represent significant difference between vaccinated and naïve groups.