Mitochondrial blue native gel (BNG) electrophoresis in C. elegans complex I RNAi knockdown strains.
figure
posted on 2009-08-12, 02:29 authored by Marni J. Falk, Julie R. Rosenjack, Erzsebet Polyak, Wichit Suthammarak, Zhongxue Chen, Phil G. Morgan, Margaret M. Sedensky(a) Identification of RC complexes in N2 (wildtype) mitochondria. (b) BNG electrophoresis of mitochondria isolated from RNAi-generated complex I subunit and assembly factor knockdown strains. All lanes contain 200 mg isolated mitochondrial protein, 3:1 Triton X:protein ratio, 8∶1 Dye/Triton X ratio, 4–12% gradient. Quantitation of individual complex content in each RNAi strain relative to wildtype is provided in Table 3.
