Mitochondrial ROS generators induce hsp-6::gfp.

A. The hsp-6 reporter strain (Phsp-6::gfp) was induced when exposed during larval development to paraquat (0.5 mM), antimycin A (0.25 µM), or rotenone (0.25 µM). A ROS generating point mutation (qm150) in the ETC complex III gene isp-1 also induced the hsp-6 reporter. Scale bar: 200 µm. B. Acrylamide, whose oxidative stress generating activity has not been linked to mitochondrial metabolism, induced the hsp-6 reporter weakly, but paraquat induction was considerably stronger. Columns represent pooled normalized values of three independent experiments plus standard error of the mean (SEM). Numbers in or on columns indicate the number of analyzed animals (n_total = 234). ***: p<0.0001; Kruskal-Wallis test plus Dunn's Multiple Comparison Test.