Micro-CT and bone histomorphometric analyses of cortical bone (A, B) Micro-CT analysis.

Micro-CT images of mid-diaphyses of femurs (A) and cortical thickness, total tissue volume, and bone marrow volume (B) in male wild-type mice (wt) and BCL2 transgenic mice (tg) at 10 weeks [wt, 17 mice; tg, 7 mice], 4 months [wt, 14 mice; tg, 10 mice], and 6 months [wt, 6 mice; tg, 6 mice] of age. Data are presented as the mean ± S.D. (C–G) Dynamic histomorphometric analysis of cortical bone at 4 months of age. C and D, Cross-sections from the mid-diaphyses of femurs of male wild-type mice (C) and BCL2 transgenic mice (D), in which calcein had been injected twice. Scale bars = 0.5 mm. E–G, Mineral apposition rate (MAR) (E), double-labeled surface (dLS/BS) (F), and bone formation rate (BFR/BS) (G) in the endosteum (En) and periosteum (Pe) at the mid-diaphyses of femurs of wild-type mice (w, blue) and BCL2 transgenic mice (t, red). Data are the mean ± S.D. of 10 mice. *vs. wild-type mice. *, ♯, $ P<0.05; **, ♯♯ P<0.01; ***, ♯♯♯ P<0.001. (H) Comparison of the serum osteocalcin level in male four wild-type mice and five BCL2 transgenic mice at 4 months of age. Data are presented as the mean ± S.D. *vs. wild-type mice. **P<0.01.