Methylation status of the pseudo-WT ICR' fragment in somatic and germ cells (line 29).

(A) Partial restriction enzyme map of the ICR' (pseudo-WT) transgene. (B–D) Southern blot analysis of the transgene in F1-3 generations. Genomic DNA was prepared from a tail-tip of TgM (pseudo-WT TgM) and the 3' portion of the transgenic H19 ICR' was analyzed (BamHI+HhaI) as described in the legend to Figure 2B and C. (E) Genomic DNA was prepare from testis of the TgM and the 3' portion of the transgenic H19 ICR' was analyzed (BamHI+HhaI) as described in the legend to Figure 2B and C. (F) Pedigree depicting a paternally-methylated pseudo-WT ICR' transgene is shown as described in the legend to Figure 2G.