Metastasis in nude mice.

(A) HE staining of brain and lung tissues. Arrows point to pathological mitotic figure. (B) X-ray scanning of bone lesions in nude mice. (C) HE staining, TRAP staining and immunohistochemical staining of tibias and femurs. (D) Metastatic incidences in the bone, lung and brain, as the percentage of the nude mice that show the lesion. “n” indicates the number of nude mice in each group.