Metalloprotease inhibitors GM6001 and Ro 31–9790 inhibit PMA/Iono- and ATP-induced shedding of CD62L on MRL+/+ T lymphocytes.
figure
posted on 2012-12-21, 00:02 authored by Sylvain M. Le Gall, Julie Legrand, Mohcine Benbijja, Hanaa Safya, Karim Benihoud, Jean M. Kanellopoulos, Pierre BobéSpleen cells from MRL+/+ and MRL/lpr mice (n = 5 mice/group) were preincubated with or without 10, 25, 50, 100 µM GM6001 or 3, 30, 100 µM Ro 31–9790 for 15 min at 37°C, and then either left unstimulated (□) or stimulated with 500 µM ATP (▴) or 5 ng/ml PMA plus 0.5 µg/ml Ionomycin (○) for 45 min at 37°C. Cells were subsequently stained with anti-CD90 and anti-CD62L mAb. Cell surface expression of CD62L was assessed in the gated CD90+ T cells by flow cytometry.
