Measurement of cytokines in immDCs.

The concentration of various cytokines (MIP- 1alpha, MIP- 1beta, IL-6, IL-10 and TNF- alpha) in the supernatant of immDCs after incubating with either Nef (100 ng/ml), gp120 (4 µg/ml) or a combination of both Nef (100 ng/ml) and gp120 (4 µg/ml) in separate sets of experiments were measured. Data represent the mean±SEM (n = 3). Statistical analysis was performed using Student' t-test, with the levels of significance defined as p*<0.05 and p**<0.01.