Mass spectrometry maps Protein C to FibA.

Domain architecture of the 744 amino acid (aa) FibA preprometalloprotease as predicted by SMART (http://smart.embl-heidelberg.de) [54]. ss: signal sequence (aa 1–24); FTP: Fungalysin/Thermolysin Propeptide PFAM domain (aa 100–149); Peptidase _M4/M4_C: peptidase family M4 and M4-Cterminal PFAM domains (aa 218–518); PPC: Bacterial pre-peptidase C-terminal PFAM domain (aa 544–614 and 638–724). Black lines correspond to the length and sequence position of the peptides identified from mass spectrometry analysis of Protein C isolated from the spore coat (above schematic) or by immune-precipitation of the aggregated cell fraction using anti-Protein C sera (below schematic).