Mapping of the 3’ ends of the egfp transcripts from the Sf21 cells infected with different viral constructs with or without the SV40 polyA signal showing the polyA signal (AATAAA) upstream of the 3’ end of transcripts (not drawn to scale).
figure
posted on 2015-12-17, 08:10 authored by Tamer Z. Salem, Craig P. Seaborn, Colin M. Turney, Jianli Xue, Hui Shang, Xiao-Wen Cheng* indicates alternative 3’ ends. // indicates sequences omitted.
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polyhedrin promoterpolyhedrin promoter activitygene expressionbaculovirus expression vector systemsSV 40 polyA increases mRNA levelsegfp mRNA levelsSV 40 polyA sequenceegfpdecreases protein productionSV 40 polyAbaculovirus prototype Autographa californicavirus 40 polyadenylation signalBEVSRNA interference applications
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