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Maize HD-Zip II proteins function as transcriptional repressors and ATHB17Δ113 relieves the repression caused by endogenous maize HD-Zip II proteins.

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posted on 2014-04-15, 04:30 authored by Elena A. Rice, Abha Khandelwal, Robert A. Creelman, Cara Griffith, Jeffrey E. Ahrens, J. Philip Taylor, Lesley R. Murphy, Siva Manjunath, Rebecca L. Thompson, Matthew J. Lingard, Stephanie L. Back, Huachun Larue, Bonnie R. Brayton, Amanda J. Burek, Shiv Tiwari, Luc Adam, James A. Morrell, Rico A. Caldo, Qing Huai, Jean-Louis K. Kouadio, Rosemarie Kuehn, Anagha M. Sant, William J. Wingbermuehle, Rodrigo Sala, Matt Foster, Josh D. Kinser, Radha Mohanty, Dongming Jiang, Todd E. Ziegler, Mingya G. Huang, Saritha V. Kuriakose, Kyle Skottke, Peter P. Repetti, T. Lynne Reuber, Thomas G. Ruff, Marie E. Petracek, Paul J. Loida

(A) Cells were co-transformed with 4 µg/320,000 cells of each reporter (right side label) and an increasing amount of each HD-Zip II family member (left-to-right for each color: 0.008; 0.04; 0.2; 1; 5 µg/320,000 cells). Error bars are 1 SD. (B) Cells were triple-transformed with 4 µg/320,000 cells of each reporter (right side label), 20 ng of each HD-Zip family member and an increasing amount of ATHB17Δ113 (left-to-right for each color: 0.0, 0.3; 0.6; 1.3; 2.5 µg/320,000 cells). For a given reporter, HD-Zip II, and concentration of ATHB17Δ113, the calculation of standard error relies on the number of biological replicates and the estimated error variance derived from a one-way ANOVA.

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