M. matsumurae and its infective symptom after being inoculated with the entomopathogenic fungus.
A, the 2nd-instar nymphs settled down in clusters under the phloem; B, a newly emerged male 3rd-instar nymph; C, a cluster of the wax cocoons in which prepupae were concealed; D, newly emerged adult female; E, the infective symptom of the 2nd-instar nymphs, showing the mycelia covered the insect body, and the insect body color darkened (arrow); F, the second-instar nymphs (si) were inoculated, but the infective symptom were observed not only in this instar but also in the following instars, including the adult female (af), the male 3rd-instar nymph (ti) and the prepupae (pp) in the wax cocoon. The infected insects were easily distinguished from the population by mycelia; G, the adult female at the early stage of infection, showing the insect body did not obviously change despite the presence of some mycelia; H, the infective symptom of the adult females at the later stage of infection, showing their body color darkened.
