MMP2, MMP9, and RANKL expression in AAAs.
A. Immunohistochemical staining for indicated proteins of serial sections of aortas one week after CaCl2 treatment. Elastic van Gieson staining is also shown. SM α-actin and F4/80 were stained to locate SMCs and macrophages, respectively. Shown are representative images of 4 or more samples in each group. Scale bars, 50 µm. B. Relative positive staining area of MMP2, MMP9, and RANKL in sections from control diet and EPA diet groups. n = 4–5. *P<0.05.